I've been questioned about the thermodynamics of my siRNAs molecules. It is well described that the strand with less stability at 5' has a higher probability to be incorporated at RISC complex.
I've been using software like siDirect to design my siRNA molecules for years, they take account several algorithms (that include thermodynamics analysis) to predict best siRNA molecule to a specific target, but this software don't return the thermodynamics of the molecules.
Last week an referee requested to add thermodynamics information of my siRNA molecules in order to confirm which strand is actually been incorporated at RISC. I tried some software like SFold with no success as they only analyze one strand each time.
Does anyone have a good suggestion?
Thank you.
Hi Andrei,
I tried the software but I think I don't know how to use it. The only thing I get is a prediction of best siRNA for my sequence. When I input the duplex sequence, they software consider an 42 nt sequence not an 21 nt duplex.
But thank you anyways! I'll keep trying to use it!
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA