I'm doing an modified version of ribonuclease protection assay and besides cleaving only ssRNA, I need to cleave dsRNA too.
I found that RNAse III cleaves dsRNA into smaller fragments (
Perhaps RNase V1, Ambion #AM2275.
But unfortunately, it's no longer produced after Ambion merged with Life Technology.
I’m doing a northern blot assay to detect transfected siRNAs on cell culture lysates. Several studies describes that liquid Northern hybridization is a more sensible method to my intended assay....
08 September 2014 534 2 View
I have a 27 nt siRNA that is cleaved by DICER enzyme into a smaller 21 nt fragment. Due to physical and chemical modifications, this 27 nt will be cleaved on an exact point and will originate a...
07 August 2014 3,136 3 View
I've been questioned about the thermodynamics of my siRNAs molecules. It is well described that the strand with less stability at 5' has a higher probability to be incorporated at RISC complex....
04 May 2014 8,906 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View