Bruno Carneiro

4 Questions 6 Answers 0 Followers

Questions related from Bruno Carneiro

Is there any specific RNAse for short dsRNA?

I'm doing an modified version of ribonuclease protection assay and besides cleaving only ssRNA, I need to cleave dsRNA too. I found that RNAse III cleaves dsRNA into smaller fragments (

11 November 2014 4,150 1 View

Does anyone have experience with Biotinylated probe detection in-gel - is that possible?

I’m doing a northern blot assay to detect transfected siRNAs on cell culture lysates. Several studies describes that liquid Northern hybridization is a more sensible method to my intended assay....

09 September 2014 497 2 View

How do you differentiate a 27 nt small RNA from a 21 nt small RNA by qPCR?

I have a 27 nt siRNA that is cleaved by DICER enzyme into a smaller 21 nt fragment. Due to physical and chemical modifications, this 27 nt will be cleaved on an exact point and will originate a...

08 August 2014 3,107 3 View

How to check siRNA molecule thermodynamics?

I've been questioned about the thermodynamics of my siRNAs molecules. It is well described that the strand with less stability at 5' has a higher probability to be incorporated at RISC complex....

05 May 2014 8,881 1 View