Has anyone tried MBP (MAB386) free floating in rat brain (40um) sections?

I have fixed rat brain sections, 40um thick, cut on cryostat. The anti myelin antibody, MAB386 gives me non-specific staining in free floating but works beautifully on paraffin sections. Any...

10 October 2018 2,479 2 View

How I register my research paper and need to know citation no and h-Index ?

For NAAC I need to register my research papers in Research gate and need to know citation no and h-Index

16 December 2017 9,524 7 View

How to calculate the extended Acm line and alpha/alpha+gamma line below eutectoid temperature in Fe-Fe3C diagram (low alloy steel -C-0.2,Mn-0.8,Si0.2?

I want to calculate the equilibrium pearlite, ferrite and bainite phase fraction below eutectoid temperature using the extended Acm and alpha/alpha and gamma line

15 October 2017 2,985 3 View

Is there any reason why the MapReduce program execution time taken on Single VM is much more than the mapreduce program run on mini-cloud ?

I have created a mini-cloud of Hadoop in my lab on few machines, when I executed a mapreduce job on the mini-cloud and on single VM(independent, not in cloud) , i found that the time taken on...

03 March 2017 7,876 5 View

How to label DNA with YOYO-1?

I would like to look at the internalization of DNA-YOYO1 by one of my peptides in some cell lines. In this regard, I am looking for a detailed protocol for plasmid DNA labeling with YOYO-1...

14 November 2016 418 2 View

Why is there the differences in the genotype band intensities in gel electrophoresis . can anyone please explain ?

10 April 2016 3,786 3 View

From where I may get MO3.13, HOG cell line of oligodendrocytes?

Is there any better option than these cell lines to study oligodendrocytes in vitro? I need to create hyperglycemic and hypoglycemic condition. Then I aim to inhibit perticular protein by adding...

21 October 2015 2,204 2 View

Can any one tell me about how to learn neuronal cell culture? is there any place in india where I can get training? i

Basically I want to learn neuronal cell culture

20 June 2014 1,795 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View