Can someone explain why my A260/A280 ratio is lower than 1?

We are conducting a measurement of Ribonucleic acid from baker's yeast (S. cerevisiae) (Sigma-Aldrich R6750) concentration using Eon Biotek Microplate reader. We dilute the RNA in various...

24 January 2021 9,830 3 View

Can we do electrophoresis of RNA from baker's yeast by vertical electrophoresis (using acrylamide gels)?

I want to do an electrophoresis of RNA from baker's yeast. However, our lab only has a vertical electrophoresis, while usually electrophoresis of nucleic acid (RNA or DNA) use horizontal...

29 November 2020 7,938 5 View

whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?

whats the difference between RNA extraction(with trisol)when we want to measure long non coding RNA and when we want to measure gene expression?

07 September 2020 7,431 5 View

Has anyone had problem with single cell whole genome amplification?

Hello, I am experiencing problem with single cell whole genome amplification from 10-12 cells only 3 or 4 amplifies and I have a smear on gel electrophoresis, Can anyone help me? Best Regards

12 February 2018 3,212 5 View

Difference between Schmid factor of perfect, trailing, and leading partials?

25 January 2017 3,347 2 View

Modeling Nucleate Boiling Using ANSYS FLUENT

20 June 2016 5,466 6 View

How should I use patch function for defining liquid-vapor interface?

Hi. I am working on a simulation of evaporation in a capillary tube.I am simulating evaporation of a water droplet sitting at the end of a capillary tube inside a solid body subjected to heat...

16 June 2016 508 16 View

Can I do with COMSOL simulation of intense laser plasma interaction and I want to expose this laser-produced plasma in an elecric field?

I need COMSOL code for simulation of intense laser plasma interaction and I want to expose this laser-produced plasma in an electric field to see double layering and then seed this laser-produced...

03 April 2016 1,597 2 View

Does anyone have experience with modelling Delamination?

17 August 2014 2,359 7 View

How can I remove the staining from the background in IHC?

I am doing IHC using an ABC staining kit (centacruz), concentration of primary and secondary antibody is optimized to detect the desired protein, incubate DAB 30 min, counterstain 5 second, but...

08 March 2013 3,561 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

What is the best solvent to remove PDMS from nano particles?

I want to remove PDMS oligomers from the surface of nanolayers. I have just read a very helpful paper about the compatibility of PDMS with different solvents( Solvent Compatibility of...

24 February 2021 7,845 5 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View