What is the best way for quantification of a FlAG-tagged protein?

Anti-FLAG ELISA can be used but the problem is that we do not have an antibody against our protein. Any suggestion will be appreciated. Thank you

11 January 2021 8,458 1 View

Could Anyone suggest the protocol for staining CD 4 and CD 8 in whole blood. Which buffer is suitable for the staining?

I am staining the CD4 and CD8 markers in the whole blood sample. However, when I used the PBS buffer (pH. 7.0 to 7.4) I have no results. Then I tried 2mmEDTA PBS buffer but still, there is no...

24 March 2020 252 3 View

Where can I buy vaccinia immunoglobulin (VIG)?

Hi, I need to perform a neutralization assay for an orthopoxvirus. I thought VIG should be easily available to purchase. However, I could not find a company to purchase it from. Most people seem...

04 February 2020 6,369 2 View

How to proceed for the counting of CD4, CD8 and CD19 from whole blood sample without isolating the WBC?

I am doing experiment with BD antibody conjugated with CD4 FITC, CD8 PE and CD19 perCp. However, I am not able to detect with higher concentration also. I need some information regarding the...

23 October 2017 8,042 2 View

How to count the live and dead cell and how to distinguish except the trypan blue technique. As, I know trypan blue only stains the dead cell?

05 February 2017 2,591 3 View

What solvent (and concentration) is suitable to dissolve essential oil (water or DMSO)? Is there any complication for using it for cell test?

30 September 2015 9,883 13 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Is an issue for serial correlation when it is not found with the xtreg, fe on its lag ,but the lag become significant when you've vce(robust) std. er?

Dear Sir/Madam I have a dateset that suffers from heteroskedasticity and serial correlation. To address the serial correlation lag my variables of the fixed effect regression. I retrieve the...

15 February 2021 3,700 1 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

How to best represent qPCR DD Ct or D Ct as a box plot?

I've been reading up on how to best represent qPCR data, specially on answers given on this site by Dr. Jochen Wilhelm. I understand box plots are the way to go, but I still don't understand them...

11 February 2021 7,228 3 View

How do I keep duplicates based on specific variable in STATA?

Hello everybody. Im new to STATA and this forum but I hope you can help me. I am doing a research project where I intend to estimate the prevalence of venous thromboembolic events (vte) up to 30...

24 January 2021 665 5 View

Which is the correct method to show Standard Error in graphs for factorial experiment?

20 January 2021 917 2 View

Is it necessary to have internal standard in Bio analytical Method Validation in HPLC?

Please suggest a suitable reference from I can get information regarding the internal standards and other calculations for bioanalytical method validations for HPLC.

19 January 2021 5,680 5 View

At what step should one add internal standards in tissue sample for LC-MS?

Hi everyone, I'm doing metabolomics of placental tissue in my lab. I added 1ml of 80% MeOH along with 2 isotopic internal standards namely, phenylalanine and palmitic acid to my sample after...

18 January 2021 9,520 4 View

How to estimate the standard deviation of the carbom emission of a company producing many different products?

I am aware of the portfolio theory in general but want to know of a journal or author especialized in this issue.

14 January 2021 7,566 2 View

Standard addition calibration without blank matrix available?

I have a problem of analytical chemistry. We are working on a spectroscopy technique (FTIR) for the quantification of a mineral phase in complex matrices. We intended to use chemometric techniques...

12 January 2021 7,739 1 View