Let me tell you what i did

First i extracted 1 g of my fruit sample in 10 ml of Methanol (7) and Water (3).

After that i took 1 ml of the extract amd again added 9 ml of mathanol and water (7:3) respectively, to make it 10mg/ml.

Then i took 1ml of the (10mg/ml) extract for FC Method

My equation comes as y=0.0071X +0.2446

R Square value = 0.9987

X = 181.18micg/ml

Then what should i take M as 1 or 0.01 in the equation C= C1 x V/M.

If i take it as 1 then the total phenolics in my sample will be 181.18mg/g GAE.

But if i take it as 0.01 then the total phenolics in my sample will be 18118mg/g GAE. which keeps me in doubt.

So i am confused about the value of M.