How to calculate the percentage secondary structures of protein from the ATR-FTIR spectra?

15 April 2019 1 2K Report

I understood the characteristics which determine the secondary structural components. but how to calculate the % secondary structures? I was confused while doing multiple peak fit, one or two peaks are slightly deviating from the original fit (i.e original spectra). please help.

Vidyalatha Kolli

thank you for the reply. the paper you mentioned helped me a lot. John Clarkson

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