Vidyalatha Kolli

5 Questions 6 Answers 0 Followers

Questions related from Vidyalatha Kolli

How to calculate the percentage secondary structures of protein from the ATR-FTIR spectra?

I understood the characteristics which determine the secondary structural components. but how to calculate the % secondary structures? I was confused while doing multiple peak fit, one or two...

15 April 2019 1,718 1 View

Can anyone suggest what is the reason behind 3T3 cells growing in clumps ?

I have seeded NIH-3T3 cells in T-25 flask. After 24 h, cells start to form clumps and continued to grow in the same manner.(Cells were grown in high glucose DMEM, 10%FBS, 1% antibiotic)

01 August 2018 6,966 3 View

Getting two absorbance peaks for purified protein sample, one at 277 nm and another at 230 nm by UV spectroscopy?

hi, I am purifying the FHF2 protein by, Ni-NTA column chromatography followed by heparin column chromatography. After purification I have loaded the purified sample in SDS page gel, there is a...

19 January 2018 909 6 View

Does methylation of DNA affect the binding of ETBR??

Does methylation of DNA affect the binding of ETBR?? Single stranded plasmid synthesis can be possible?

13 September 2014 5,122 4 View

Can anyone help me troubleshoot a problem I've been having with agarose gel electrophoresis?

Agarose gel electrophoresis, I have ran (2ul sample+1ul 6X gel loading dye+ 3 ul sterile water) plasmid (6kb) sample in 1 % agarose (Low EEO grade) gel with Zip Ruler Express DNA Kb ladder (...

12 September 2014 5,352 4 View