I am doing an experiment where I am applying a drug 40 microMolar to the apical dissolved in HBSS (500mcl total), and no drug just 1.5 ml of HBSS to the basolateral and then I measure the concentrations in the basolateral taking 100mcl and replace it with 100mcl HBSS to maintain sink conditions at different time points. and finally get the concentration using HPLC.
now how to calculate the cumulative concentration in the basolateral?!
Thanks.
Hi,
At each sampling time 15% of drug that reached the basolateral compartment is discarded (100mic/1500mic) which is a significant loss. If you don't consider this, concentrations measured will decrease with time because of the dilution but also because the release rate will slow down as basolateral concentration come closer to the apical concentration. To obtain a cumulative concentration, it is quite simple you have to add the quantity of drug (mass) removed in the basolateral compartment in all previous samples except the fisrt one (Q= concentration measured at each time x 100mcl).
Hoping this will help you!
Hi Dr,
You may find these literature / papers useful:
Full-Text PDF - MDPI.com
www.mdpi.com/1420-3049/20/6/10264/pdf
by S Shityakov - 2015
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Determination of drug permeability and prediction of drug absorption ...
www.nature.com › Journal home › Archive › Protocol
by I Hubatsch - 2007
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