Hello, can you please guide us on the hydrogeling method for decellularized skin?
Dear researchers, Anticancer peptides(ACPs) with cell-penetrating properties can enter to cytoplasmic space of cancer cells and induce cell death. but, I could not find the database that...
29 January 2021 707 3 View
I want to calculate the concentration of DNA in an unknown sample. But it's buffer has a high concentration and our nanodrop don't accept that as a blank of dsDNA. Thus I dilute the buffer with...
23 January 2021 9,440 2 View
I had a sample of plasmid extracted from e.coli and wanted to check the amount of Host cell RNA impurity with a fluorescent dye. to do this, first of all, I treated the sample with DNase I. (NEB...
11 January 2021 2,608 3 View
hello I would like to ask for help regarding calculation of dilution factor to get the amount of mycotoxin per gram of sample. the extraction protocol as fellow: -ground 5 g of sample with 25 mL...
29 November 2020 1,831 15 View
Dear researchers : y-randomization is one of the special tests of machine learning approaches. I want to evaluate my developed machine learning-based models, to predict active peptides, by...
26 October 2020 1,196 2 View
I am modelling an impact on a Ti64 block. The impacting particles are rigid and the Johnson-Cook material has been used for the block. I am wondering how to calculate erosion; I want to analyze...
13 April 2020 4,509 2 View
hello, I am working on the extraction of trichothecenes from the grain of wheat and I would like to ask how is it possible to calculate the concentration of toxin ug per gram of wheat? the...
17 March 2020 7,469 3 View
How I can get source code for 802.11a PHY layer in matlab without using simulink?
27 December 2019 2,191 3 View
I want to insert my 14kb recombinant plasmid into A.Rhizogenous, what should I do during my transformation. I transformed my plasmid with freeze/thaw transformation method, but it didn't work. I'm...
03 December 2019 960 7 View
I've been measured the percent of my concentration series (R2 = 0.998) but the data weren't covered value more than 50%. Should I repeat my experiment or I just extrapolate the regression...
29 July 2019 2,899 5 View
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...
28 February 2021 552 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...
17 February 2021 384 3 View
I want to perform the synthesis of a hydrogel and I want to know if I can polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?
02 February 2021 2,106 6 View
I want to add silicon nanoparticles to my hydrogel, but when I sonicated these particles with water to disperse them in water, they agglomerated on the water's surface. How can I disperse them in...
26 January 2021 213 4 View
I am working on PVA-GMA hydrogel that is a really strong polymeric structure like rubber. Because I am in the middle of analysis, it is difficult to dissolve the gel using a common solvent. I...
20 January 2021 3,501 2 View
I am seeing non-motile black dots of almost equal size along the periphery of skin melanoma cell line A375. They are present in almost all the cells. They don't turn the media turbid. However...
18 January 2021 5,151 1 View
I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...
17 January 2021 4,170 1 View
Hello, Most hand sanitizers contain 60-80% alcohol. Alcohol destroys organisms including bacteria, fungi, protists, and enveloped viruses by breaking down the plasma membrane. In recent...
16 January 2021 6,923 11 View