Hello,
I am using Neon transfection system to transfect siRNA into suspension cell line. When calculating siRNA conc., does it have to based on tip volume or final volume. Although, i have an idea that once it is internalized, it does not matter. Therefore, it should be based on tip volume. But i would like to confirm that.
I will appeciate if anyone who is familiar with this technique, can give suggestions.
Thank you
Thank you for your response.
I have used siRNA previously in adherent cell line and have already established the conditions. I would like to use same or nearly same concentration in suspension cell line. Lets say I need to use 10nM, does that conc is calculated for volume of mixture that goes into electroporation tip or final plating volume ?
You can find the answer on page 14:
"Note: The siRNA concentration in the Neon® transfection protocol refers to
the siRNA concentration in the culture medium and not to the siRNA
concentration in the electroporation mix in the Neon® Tip."
So it's the final concentration in the medium, not the tip.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line