Yiqi Huang

6 Questions 6 Answers 0 Followers

Questions related from Yiqi Huang

Does phenol-chloroform DNA extraction remove bacteria contamination?

I found out that the water I used to dissolve my DNA after phenol-chloroform extraction was contaminated with bacteria (not sure which, but likely E.coli used for cloning). Can I remove these...

22 January 2024 7,919 2 View

Collagen-coated transwell: does it affect mass spectrometry?

Hi people! I was planning to run some proteome analysis on ALI cultures, but these cells are growing on collagen-coated transwells. I wonder if the collagen coating will conatminate the mass spec...

29 July 2020 6,736 4 View

Does the titre of flavivirus drop when stored in supernatant at -80?

I work with the vaccine strain of yellow fever (YF17D) as well as the YF17D-Venus strain that expresses Venus upon replication. They are passaged in BHK21 cells in our lab. Right now we don't have...

10 May 2019 1,979 5 View

What's the difference between heat- and UV- inactivation of viruses?

Hi there! I work with the yellow fever vaccine strain 17D and I always inactivate the virus at 56 degrees for 1h as a control group. However, I've also seen UV inactivation and in one of the...

01 May 2019 4,360 4 View

What's the actual difference between buffer R and T in Neon transfection system?

Hello people! I'm trying to transfect my monocyte-derived dendritic cells(MoDC) with some siRNA using Neon electroporation system. The protocol recommended to use buffer T for primary...

24 April 2019 5,564 1 View

Fixed/Permed cells left overnight at room temperature: can I still use them for FACS?

Hi folks! I have come across an accident when I did my FACS intracellular staining: I completed the whole staining process but had to leave towards the end of the experiment, so I handed it over...

29 March 2019 1,812 2 View