I have carried out i-t responses for 1, 2.5 & 5 µM concentrations for my analyte. Below 1 micro molar the i-t responses were not significant. By CV I could only carryout samples upto 10 micro molar.
How to calculate Sensitivity and LOD from Amperometry?
Hello Sureshkumar,
Essentially you are looking to plot the current response against the concentration. If you are operating in the linear range you can then derive the linear equation for the response and the gradient will describe your sensitivity (e.g., 15.8 µA / 1 µM).
Unfortunately there are a few different ways to determine the LOD. You may find the following discussion on ResearchGate interesting:
https://www.researchgate.net/post/How_to_calculate_limit_of_detection_limit_of_quantification_and_signal_to_noise_ratio
Furthermore, see this article for a good overview of the 'limit of blank', 'limit of detection' and 'limit of quantification':
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2556583/
Hope it helps,
Jules
suresh kumar,
you can find the answers in these articles
Sensors and Actuators B: Chemical 230, 140-148, 2016
Journal of The Electrochemical Society 164 (5), B3036-B3042 , 2017
3S/m & 10S/m from these eqn, you can calculate LOD & LOQ, where S is standard deviation & m is slope of the curve
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