I'm a first year PhD student (1 month in) looking into the activity of an oxidation electrocatalyst, similar to TEMPO. I would like to see what kind of conversion rate I can achieve over a couple of hours. My cell is always made up to 3mL and I use around a 2mM concentration of my catalyst. What kind of ballpark concentration should I be using for my substrate? My CV analyses have been using around 200mM substrate when looking into catalyst diffusion coefficients, as to not limit the current to the diffusion of the substrate, but I have no idea if this is very high, very low, or just right for an amperometry experiment.
Thank you in advance!
Supporting electrolyte concentration: 0.15-0.25M
Solvent used: DCM
Please look at the following below links which may help you in your analysis:
http://www.scielo.br/pdf/jbchs/v8n6/a02v08n6.pdf
http://www.electrochemsci.org/papers/vol9/90200990.pdf
https://pdfs.semanticscholar.org/e111/833d378fa357862358453ab754800fcd5e4b.pdf
https://www.hindawi.com/journals/js/2017/7498945/
Thanks
Isam Eldin Hussein Elgailani That's helpful; thank you! I'll take a look.
Dear Scott Place ,
note, if possible, the WE[1], CE (and RE[1] ?) electrodes' materials of your cell, since the amperometric transduction is based on the oxidation and reduction of electroactive species on the biosensor surface (WE)[3].
Also, show, please, some of your CV(s).
1. High Accuracy Amperometric Sense and Control Circuit for Three-electrode Biosensors https://romjist.ro/content/pdf/enache.pdf
2. Chapter 4 - Graphene Functionalization for Biosensor Applications https://www.sciencedirect.com/science/article/pii/B9780128029930000046
3. Sequentially multiplexed amperometry for electrochemical biosensors https://www.sciencedirect.com/science/article/pii/S0956566318304810
Dear Scott Place,
Generally amperometry techniques use 10^-8 to 10^-4 mol dm^-3 but can be as low as 10^-15 mol dm^-3
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