How to calculate N/P ratio for plasmid DNA/branched PEI complex?

16 April 2019 0 666 Report

I need to prepare plasmid DNA polyplex with branched PEI, with different N/P ratio. My plasmid is around 6kb. How do I calculate the N/P ratios, the amount of plasmid DNA and the amount of bPEI to be added for a particular N/P ratio?

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