Shlok Jindal

3 Questions 3 Answers 0 Followers

Questions related from Shlok Jindal

How to calculate N/P ratio for plasmid DNA/branched PEI complex?

I need to prepare plasmid DNA polyplex with branched PEI, with different N/P ratio. My plasmid is around 6kb. How do I calculate the N/P ratios, the amount of plasmid DNA and the amount of bPEI to...

16 April 2019 666 0 View

What is GC clamp and why should PCR primers not contain GC clamp?

I am desinging PCR primers. When I submit my primer sequence in Sequence MAnipulation Suite to check the different paramenters of my primers, the software gives warning that there are more than 3...

19 August 2018 7,242 5 View

How to decide the concentration range for determining the IC50 value of a nanoparticle by MTT assay?

I want to determine the IC50 value of a nanocomposite (which I have synthesized) by MTT assay. How do I decide that what particular concentrations of the nanocomposite should I take for the MTT assay?

01 August 2018 4,237 6 View