So I did the BCA assay and came up with these concentrations/ul:
Sample 1 = 2.9 ug/ul, Sample 2 = 2.5 ug/ul, Sample 3 = 3.5 ug/ul, Sample 4 = 2.7 ug/ul.
I want to calculate how much of each to load in the gel for blotting.
Suppose I will put total volume of 12 ul in each well, of which 3 ul will be 4xSB.
How to calculate the volume of each sample? I can't get it.