Ahmad El-Hamamy

8 Questions 10 Answers 0 Followers

Questions related from Ahmad El-Hamamy

Trouble with Mouse ICV stereotaxic injections where the dye doesn't diffuse to the other ventricle, how can I find out the problem?

I recently started learning stereotaxic injections in mice, we will be targeting the Medulla but I started with practicing ICV to get used to the process using Evan's Blue Dye 1:100, and I had a...

04 November 2021 5,539 3 View

Trouble with setting up a PCR cycle for a mouse strain and getting results, what should I do?

Hi all, I have a new strain of EZH2 floxed mice that I need to genotype because I will create KO mice later, no one in our lab deals with it so there is no program for it in our...

10 June 2021 9,246 6 View

How to use ImageJ to assess infarct size in mice brain after cresyl violet staining?

Hello everyone, I have some brain slides that I CV stained, and I need to learn how to assess infarct volume and quantify it comparing it to the other hemisphere or my controls. I looked online...

22 March 2021 6,203 2 View

Having trouble mounting the sections on the slides for IHC staining, what solution should I use?

Hello everyone, I just started learning IHC staining and I have some frozen sections to practice with in a 96-well plate. I'm having trouble mounting the sections on the slide, a colleague told me...

09 February 2021 7,158 3 View

How to use Image Lab to take an image for the bands and the ladder in the same image merged together in Western Blot?

Hello everyone. I was trying to use Image Lab software to take an image of both the ladder and the samples merged together in one image to see the position of the bands according to the ladder MW,...

05 February 2021 10,510 2 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

28 January 2021 5,612 3 View

I have some "stupid" questions about the Western Blot, can you help me?

I started learning the Western Blot a few days ago and I have an okay understanding of the process, but there are a few steps I don't understand how to do by myself from scratch. I want to...

26 January 2021 6,817 8 View

How to calculate how much sample to load in the Western Blot after BCA?

So I did the BCA assay and came up with these concentrations/ul: Sample 1 = 2.9 ug/ul, Sample 2 = 2.5 ug/ul, Sample 3 = 3.5 ug/ul, Sample 4 = 2.7 ug/ul. I want to calculate how much of each to...

25 January 2021 5,447 4 View