Generally, to maintain ecological status, five factors are considered for a river ecosystem:
1. flowing water that is mostly unidirectional
2. a state of continuous physical change
3. many different (and changing) microhabitats
4. variability in the flow rates of water
5. plants and animals that have adapted to live within water flow conditions.
During water distribution of trans border water resources, only water is considered irrespective of water ecology. If we want to consider river ecology, especially for maintaining fish population, and want to estimate minimum water flow, what procedure may be adopted to identify minimum required flow?