Alaa Youssef Farag

5 Questions 6 Answers 0 Followers

Questions related from Alaa Youssef Farag

Can miRNAs affect transcription but not translation of their target genes?

I understand that miRNAs can directly affect protein levels without a noticeable effect on the mRNA level of their target genes. However, my question: can the opposite happen? Can a gene that was...

26 April 2020 5,115 0 View

What are the bright blue/white and black dots in my DAPI stained cells in the transwell migration assay? which cells should i count?

I am new to fluorescence microscopy and i am doing a transwell migration assay for two cell lines (HepG2 and SNU449). I am still optimizing the technique with non-treated cells. My procedure is as...

30 December 2019 8,249 1 View

I am getting no amplification curve at all and weird melting peaks?

I would really appreciate your support as this is my first introduction to the real time PCR. I am performing relative gene expression using power up syber green and on a biorad cfx instrument....

13 January 2019 9,062 5 View

How to optimize syber green RT-qPCR ?

I am very new to real time PCR (I did my first run today) and i got nothing so i will really appreciate your patience and detailed answers. As a beginning i wanted to test the expression of one...

08 January 2019 3,132 4 View

Buying mirNA mimic and inhibitors from qiagen ?

I want to run a transfection experiment using mimics and inhibitors of a specific miRNA. Since it is my first experience in the master's thesis, I don't know which companies are the best. in the...

13 September 2018 9,496 3 View