If i am Knowing the confluency of cell culture, is it possible to calculate the doubling time of cells.
Normally , in case of 2 times dilation like (5/5) the next day it becomes confluent. So by that observation, I think the cell doubles in 24 hours. But its sure the time may differ cell to cell...
Thank you..
Cell doubling time can be calculated in the following way using growth rate (amount of doubling in one unit of time)
Growth rate:
gr = 1n ( N(t)/N(0)) / t
N(t) = the number of cells at time t
N(0) = the number of cells at time 0
gr = growth rate
t = time (usually in hours)
Doubling time:
t{doubling time}= 1n(2) / growth rate
Cell doubling depends on many factors, including culture conditions and viability of cells. The best way to monitor cell doubling is to count them on a daily basis at fixed time. For adherent cells, you can seed a fraction of cells in small dishes /flasks and use them as indicators of the whole cell line used. If the cells are in suspension this step is much easier as you can keep them in one large vessel: mix the cells by shaking the culture vessel, take a small amount from the middle of the cell culture medium and count in the hemocytometer. Best.
The population doubling time or generation time (tg) is usually calculated during the logarithmic phase of growth. It specifies the time (t) in hours needed by the culture to double its cell number.
tg = t / n
with n = number of population doublings
The number of doublings (n) can be calculated using the following formula: n = (log N – log N0) / log 2 N: total viable cells at harvest
N0: total viable cells at seed
log 2 = 0.301
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