How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

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In a physiology textbook treatment of reproduction, they describe the Corpus Luteum as yellow as a result of the color of cholesterol. Yet, pure cholesterol is white, and has no structures that...

15 November 2020 7,398 3 View

Anyone written or used code to do numerical integration of borehole curves?

Just learning Matlab and know a little Python. I have some borehole curves, trace element data in ppm and I want to estimate the volume of of the element over a depth interval using numerical...

28 March 2020 9,572 3 View

Viral load hand washing: why not twice instead of once for 20 sec?

Why isn't washing twice a recommendation? When you do an extraction, say of lipid fractions into organic solvent, or really any separation, the increase multiple times is far more effective than...

18 March 2020 5,600 3 View

In which salt's 0.5 Molar aqueous solution at 35 degrees Celsius would Ammonia gas at 1.4MPa have the least solubility? NH3 Molality at 14 or below?

Looking for the salt with the greatest ability to effect the release of Ammonia gas from aqueous ammonia at a pressure of about 1.4MPa (i.e. salt not precipitating out of solution) and at a...

23 February 2020 4,530 3 View

What is the best statistical method to use for analyzing a questionnaire given multiple times?

For clarification, I am doing research on asynchronous mathematics video collaboration between students of different countries. In order to measure this, I designed a 20-some question survey using...

28 January 2020 3,605 3 View

How to find correlation between likert scale and categorical variables?

I am doing a survey regarding people's awareness on fire safety. In Part I, I gather information like gender (2 categories), education levels (5 categories) etc. In Part II, I set 9 questions...

29 December 2019 4,308 3 View

Any methods to make activated carbon and sand into a non-segregating mixture?

just wondering if there are any potential methods to make activated carbon (either granular or powdered) and sand into a non-segregating mixture? open to suggestions.

25 September 2019 284 3 View

Scheduling algorithm for mult-users tasks in a fog / cloud environment?

We consider a 5G architecture consisting of 3 layers: the cloud layer, fog layer and an edge layer (user layer). Our goal is to set up a scheduling algorithm that involves joint processing of...

02 May 2019 1,366 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View