Does growing bacteria in serum affect the quality of genome sequencing?

Hi, My lab is getting prepped up to do genome sequencing of several S. pneumoniae and N. meningitidis isolates. We got far better growth for S. pneumoniae with 5% Sheep blood serum in Todd-Hewitt...

06 July 2015 1,141 6 View

Which is better - a single-quenched probe or double-quenched probe for multiplex qPCR ?

My lab is planning to work on multiplex qPCR to detect multiple organisms from clinical sample. We are now ordering the primers and probes. However, I found single quenched probe and...

31 December 2014 7,131 2 View

No title

11 December 2014 2,410 3 View

Which PCR model is better: ABI GeneAmp 9700 Dual 96-well or ABI Proflex Dual 96-well?

I need to know which one model is better between ABI GeneAmp 9700 Dual 96-well and ABI Proflex Dual 96-well. I have little experience on with the GeneAmp 9700, but it's not that good. I found...

10 November 2013 1,248 4 View

Need help for psipred tool.

Do anyone have any detail experience on psipred web server? I need help on this matter. I have a few unannotated protein sequences, for which I want to do functional annotation. I have already...

04 May 2013 501 5 View

Free software for ILD test

I have several analyses including distance estimation and also phylogenetic tree based on DNA sequences data. I used MEGA 5.05 to do these. Now I need to do ILD test and Templeton Test to...

06 July 2012 8,285 12 View

Explanation for Phylogenetic tree

I am working on some sequences from five different region ITS, IGS, MatK and XTH of two plant genera I have built phylogenetic trees using Maximum parsimony and Maximum Likelihood method by MEGA...

08 September 2011 802 3 View

Need to know the best way for washing filtering crucible...

I am doing Lignin Estimation by Kalson Method. There has been some problems with washing, which causes disturbances in my results. Several months ago, I found out from a website that filtering...

05 June 2011 7,369 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View