Does growing bacteria in serum affect the quality of genome sequencing?

Hi, My lab is getting prepped up to do genome sequencing of several S. pneumoniae and N. meningitidis isolates. We got far better growth for S. pneumoniae with 5% Sheep blood serum in Todd-Hewitt...

06 July 2015 1,141 6 View

Which is better - a single-quenched probe or double-quenched probe for multiplex qPCR ?

My lab is planning to work on multiplex qPCR to detect multiple organisms from clinical sample. We are now ordering the primers and probes. However, I found single quenched probe and...

31 December 2014 7,131 2 View

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11 December 2014 2,410 3 View

Which PCR model is better: ABI GeneAmp 9700 Dual 96-well or ABI Proflex Dual 96-well?

I need to know which one model is better between ABI GeneAmp 9700 Dual 96-well and ABI Proflex Dual 96-well. I have little experience on with the GeneAmp 9700, but it's not that good. I found...

10 November 2013 1,248 4 View

Need help for psipred tool.

Do anyone have any detail experience on psipred web server? I need help on this matter. I have a few unannotated protein sequences, for which I want to do functional annotation. I have already...

04 May 2013 501 5 View

Free software for ILD test

I have several analyses including distance estimation and also phylogenetic tree based on DNA sequences data. I used MEGA 5.05 to do these. Now I need to do ILD test and Templeton Test to...

06 July 2012 8,285 12 View

How to build combined data matrice?

Could anybody help me with a building up procedure for combined data matrix? I need to combine two data matrices from DNA sequences for phylogenetic analysis. I am using MEGA for analysis.

06 July 2012 7,163 7 View

Explanation for Phylogenetic tree

I am working on some sequences from five different region ITS, IGS, MatK and XTH of two plant genera I have built phylogenetic trees using Maximum parsimony and Maximum Likelihood method by MEGA...

08 September 2011 802 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View