Arif Mohammad Tanmoy

9 Questions 14 Answers 0 Followers

Questions related from Arif Mohammad Tanmoy

Does growing bacteria in serum affect the quality of genome sequencing?

Hi, My lab is getting prepped up to do genome sequencing of several S. pneumoniae and N. meningitidis isolates. We got far better growth for S. pneumoniae with 5% Sheep blood serum in Todd-Hewitt...

07 July 2015 1,196 6 View

Which is better - a single-quenched probe or double-quenched probe for multiplex qPCR ?

My lab is planning to work on multiplex qPCR to detect multiple organisms from clinical sample. We are now ordering the primers and probes. However, I found single quenched probe and...

01 January 2015 7,208 2 View

Which one is better among Qubit® 2.0 Fluorometer, NanoPhotometer® P-Class and NanoDrop 2000c..for estimation of Nucleic acid concentration ?

Among these three DNA-quantification system, I have experience with NanoDrop (Thermo). However, I don't have any experience with others. According to the supplier's info, Qubit® 2.0 Fluorometer...

12 December 2014 2,530 3 View

Which PCR model is better: ABI GeneAmp 9700 Dual 96-well or ABI Proflex Dual 96-well?

I need to know which one model is better between ABI GeneAmp 9700 Dual 96-well and ABI Proflex Dual 96-well. I have little experience on with the GeneAmp 9700, but it's not that good. I found...

11 November 2013 1,312 4 View

Need help for psipred tool.

Do anyone have any detail experience on psipred web server? I need help on this matter. I have a few unannotated protein sequences, for which I want to do functional annotation. I have already...

05 May 2013 563 5 View

How to build combined data matrice?

Could anybody help me with a building up procedure for combined data matrix? I need to combine two data matrices from DNA sequences for phylogenetic analysis. I am using MEGA for analysis.

07 July 2012 7,213 7 View

Free software for ILD test

I have several analyses including distance estimation and also phylogenetic tree based on DNA sequences data. I used MEGA 5.05 to do these. Now I need to do ILD test and Templeton Test to...

07 July 2012 8,368 12 View

Explanation for Phylogenetic tree

I am working on some sequences from five different region ITS, IGS, MatK and XTH of two plant genera I have built phylogenetic trees using Maximum parsimony and Maximum Likelihood method by MEGA...

09 September 2011 857 3 View

Need to know the best way for washing filtering crucible...

I am doing Lignin Estimation by Kalson Method. There has been some problems with washing, which causes disturbances in my results. Several months ago, I found out from a website that filtering...

06 June 2011 7,430 0 View