I am performing cell cycle assays using SH-SY5Y cells and comparing different treatments to no treatment in order to investigate potential effects on the cell cycle. I can see obvious differences in the distribution of peaks, but I am not sure about how to set the markers to distinguish different phases of the cell cycle. These are freely adjustable and I cannot seem to find any info online about how to best set them. Are there standard values of DNA content index that represent the different cell cycle phases? How can I set them to best represent the actual G0/G1, S phase etc? (See picture attached) thanks a lot!!