I have a balanced panel data of 12 countries and nine indicators. which methodology would give better results and what is the rationale of using it?

I have a balanced panel data of 12 countries (5 countries are developing and rest are advanced economies) and nine indicators. which methodology would give better results and what is the rationale...

03 September 2020 5,417 2 View

Other than the analytical scoring, what is the best way to make the scoring of EFL students' writing performance more objective?

I am working on a study in which I need to adequately evaluate my EFL students' writing performance.

10 December 2018 2,769 49 View

How do I find the ''dopant percentage (at.%)'' during the manufacturing of doped ZnO nanoparticles by sol-Gel Process??

I want developed Ag-doped-ZnO nanoparticles by sol-gel process with different at% (0.1,0.2,0.3) of dopant material so, how can I find this at% of dopant material ?? Thank You.... Regards.

01 December 2018 7,263 3 View

What should be the control variable?

12 November 2018 8,319 2 View

What are the potential health risks due to massive distributed antenna systems in our Neighborhoods? any tips for protection ?

A huge number of antennas (transmitters and recievers devices) systems popping up over the bulding in our neighborhoods, for the internet and communication services. what is the possible risk of...

27 September 2018 7,196 9 View

What precautions should be used when pouring concrete in hot weather ?

Placing Concrete in Hot Weather Caution needs to be applied when placing concrete in hot weather what are they?

07 July 2018 9,165 13 View

What do you suggest of the following to increase the punching shear of a RC slab with the reason?

1_ increasing the slab thickness 2- using drop panel 3- using shear reinforcement 4- increasing the concrete compressive strength:

07 July 2018 5,327 10 View

Do you have training courses to make models for my thesis on climate change and food security in Senegal?

Currently I am a student in Master II at the Faculty of Economics and Management of the University Cheikh Anta Diop Dakar.I finish this higher level with a dissertation whose theme is "the impacts...

01 June 2018 8,422 4 View

What is the best method to extract RNA (including miRNA) from plasma if it is needed for NGS RNA sequencing?

Dear all, Few years ago, I used miRCURY Biofluids from Exiqon for extracting RNA from plasma for detecting miRNA by qPCR. However, this product is currently discontinued since Exiqon is now...

23 May 2018 6,880 4 View

Can you freeze whole blood, and then subsequently isolate PBMCs?

Hi, we are working in a very resource limited setting where the lab resources on site are very basic. We are doing serial blood sampling for drug concentrations, which includes overnight samples....

08 May 2018 746 11 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View