How to avoid rna degradation while performing placental tissue rna extraction?

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Prem Kumar G

Hi sree,

At first treat all your materials with DEPC (Diethyl PyroCarbonate) and autoclave it. During this step most of the RNase are degraded. All the best.

Sreenithi Santhakumar

but I have tried with Rnase zap solution.it didn't work well. I'm not sure whether tissue kept it rna later for long time may cause degradation.