I am wondering what some quantifiable methods to assess plant tissue culture performance could be. I have seen some publications use destructive metrics, such as shoot length or culture weight, but it is difficult to perform these measurements without interrupting the culture sterility. I have also seen some metrics like # of new shoots produced, but I feel this is largely based on the way the explant is prepared going into culture.
I was wondering what are some metrics you use to assess plant performance in different media and conditions. We are doing protocol research to figure out the best culture conditions to grow the plants, but our notes and analysis feel very subjective, and we are not relying on any quantifiable metric. I have been thinking of developing a scoring system, but wanted to see what you all thought!
The protocol of work in the field of tissue culture is different but the most important stage of selection of the medium used and determine the organ to be grown in the case of multiplication is the target quantitative and for this purpose and the author of the growth organizations and energy security by sucrose and the center of acidity. Also we can grow young shoots to obtain mature buds and secure middle Good for the growth of pollen and to overcome the weakness of the growth of the pilot tube when the plant suffers from a low rate of anthrax. And we get a qualitative result. I hope my answer is convincing
You could use digital image analysis, for example like in the attached paper.
I'm working of breeding of vegetables, hybridization , backross ، Self-vaccination and production new varieties of vegetables.
The digital imaging is a good idea. Could you do biomass if you weigh the tube and media prior to culture?
Anna Schwabe Thank you for your suggestion.
I was wondering about this, will we see an increase in the biomass? Or would we see a relevant transfer of mass from the nutrients being consumed in the media.
As I posted it, that's what I was wondering too. If you did both imaging and mass data, you could answer that question and publish it so we know! I would think you would see an increase in biomass, since photosynthesis uses a CO2 which isn't in the media, right?
Anna Schwabe I would love to! I will do some mass experiments in the following weeks and would be happy to share the data.
We work on a photoheterotrophic system now so the explants do not photosynthesize, but get their carbon from the sugar. I have weighed the explants themselves out of the culture tubes and they definitely increase in mass, but I cannot maintain the sterility with this measurement.
WE CAN COUNT THE NUMBER OF LEAVES AND NEW BUDGETS PER UNIT OF TIME. MEASUREMENTS CAN ALSO BE CARRIED OUT THROUGH THE PROCESSING OF IMAGES TAKEN IN IN VITRO CULTIVATION TO EVALUATE THE LENGTH AND DIMENSION OF THE PLANTS OBTAINED AND THEIR ROOTS. IT IS ALSO POSSIBLE TO TAKE THE WEIGHT OF THE CULTIVATOR BOX IN SEWING DURING THIS PROCESS
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