Josh Paster

3 Questions 17 Answers 0 Followers

Questions related from Josh Paster

Can anyone help with problems with density-gradient (Percoll) PBMC isolation?

I am experiencing large variations in our Peripheral Blood Mono-nuclear Cell (PBMC) isolation from NHP whole blood. We are currently using Percoll diluted to 60 % to isolate, and the results have...

15 January 2015 3,750 12 View

Does anyone have any experience with sensitizing mouse with Ovalbumin (OVA) protein?

I am trying to sensitize a B6 mouse against the Ovalbumin protein, so I was going to inject the protein mixed with some sort of adjuvant (Freund's most likely). Does anybody have experience with...

05 September 2014 7,933 4 View

How to assess health/performance of plant tissue cultures

I am wondering what some quantifiable methods to assess plant tissue culture performance could be. I have seen some publications use destructive metrics, such as shoot length or culture weight,...

01 January 1970 4,396 9 View