Can VSV proteins be shared among viruses?

Dear biologists, I am wondering when we co-infect insect cells with two viruses (BacMAM viruses), only one of them containing VSV gene. Would VSV-G protein integrate onto both viruses produced...

10 November 2017 1,886 0 View

How to improve PCR fidelity while using DMSO to overcome GC rich template PCR?

Hi friends, I am trying to clone some GC rich fragments. The PCRs work well while I use 5% DMSO in the reactions, hardly any band without DMSO. The problem is that the DMSO inclusion largely...

09 October 2017 6,420 3 View

How can I find out the transcription start site (TSS) of gene CDKN1A?

Dear friends, May you let me know how can I find out the transcription start site of gene CDKN1A from any database or any public resource? Thank you. ziguo

06 July 2017 2,235 2 View

Chromosome associated RNA (caRNA), where I can find database for none coding RNA including caRNA?

06 July 2017 7,182 1 View

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11 December 2016 6,295 1 View

Any anti monomeric EGFP antibody available?

06 July 2016 3,894 5 View

What special attention should I pay for purification of recombinant type II restriction EcoRV?

05 June 2015 1,556 4 View

Can ProA-immunoglobulins affinity be directly used for western?

I have a ProA tagged protein in yeast. I wonder if I can use yeast crude lysate in SDS PAGE, then visualize ProA by anti-mouse IgG-peroxidase conjugate as western analysis? Or I have to do it in a...

03 April 2015 5,188 1 View

How can we increase the solubility of E. coli overexpressed protein?

We are trying to purify an artificial protein. Very good expressed, but mostly in the spun down pellet. If anyone has experience to make it a bit more soluble, with a kind of mild buffer,...

31 December 2014 5,830 8 View

How can we perform migration of peptide correctly in SDS-PAGE?

11 December 2014 6,034 8 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View