How can I find out the transcription start site (TSS) of gene CDKN1A?

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Can VSV proteins be shared among viruses?

Dear biologists, I am wondering when we co-infect insect cells with two viruses (BacMAM viruses), only one of them containing VSV gene. Would VSV-G protein integrate onto both viruses produced...

10 November 2017 2,087 0 View

How to improve PCR fidelity while using DMSO to overcome GC rich template PCR?

Hi friends, I am trying to clone some GC rich fragments. The PCRs work well while I use 5% DMSO in the reactions, hardly any band without DMSO. The problem is that the DMSO inclusion largely...

09 October 2017 6,542 3 View

Chromosome associated RNA (caRNA), where I can find database for none coding RNA including caRNA?

Dear all, I am trying to find some sequence information about chromosome associated RNA. Please may you let me know where should head? Thank you.

06 July 2017 7,325 1 View

Coli strain for plasmids with R6Kγ origin and Reduces plasmid recombination/increases plasmid stability?

Dear friends, Does anyone knows any Coli Strain available for plasmid with R6Kγ origin (pir gene) and with reduced recombination to increase plasmid stability? I am currently experiencing a very...

11 December 2016 6,427 1 View

Any anti monomeric EGFP antibody available?

Dear All, I am aftering anti-monomeric EGFP antibody for western blotting analysis. Any friend knows where I may buy such antibody, please? Thank you.

06 July 2016 4,019 5 View

What special attention should I pay for purification of recombinant type II restriction EcoRV?

Hi experts, I need a lot EcoRV restrict enzyme for routine usage. I prepare to make by my own. As restricts are not normal protein, do I need special tricks to deal with the over-expressed enzyme...

05 June 2015 1,672 4 View

Can ProA-immunoglobulins affinity be directly used for western?

I have a ProA tagged protein in yeast. I wonder if I can use yeast crude lysate in SDS PAGE, then visualize ProA by anti-mouse IgG-peroxidase conjugate as western analysis? Or I have to do it in a...

03 April 2015 5,317 1 View

How can we increase the solubility of E. coli overexpressed protein?

We are trying to purify an artificial protein. Very good expressed, but mostly in the spun down pellet. If anyone has experience to make it a bit more soluble, with a kind of mild buffer,...

31 December 2014 5,954 8 View

How can we perform migration of peptide correctly in SDS-PAGE?

Dear All, I am trying to detect synthesised peptides with SDS-PAGE. The peptides migrate with a big difference (larger) in SDS-PAGE gel in comparison with their real molecular weight. And they...

11 December 2014 6,171 8 View

How can I decrease lysate viscosity for isolation of DNA/protein complex from cell disruption?

Dear all, I am going to isolate a DNA/Protein complex from insect cells. In protein purification practice, generally, we use nuclease during the cell disruption to destroy the released nucleic...

07 August 2014 3,519 6 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How to convert a privately loaded document into a public document?

I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...

05 August 2024 8,025 7 View

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

How can productivity (using the Google form link below to provide your answers) be achieved in manuscript publication?

Survey on Productivity in Journal Manuscript Publication Survey Form Link: (https://forms.gle/YRVrn8dL4WZJJ79S8 ) Dear Researcher, We kindly invite you to participate in our survey focused on...

29 July 2024 4,116 1 View

How can I find writers for drafting, submitting, and publishing papers?

Looking for paper collaboration writers： Collaboration Model 1: 1.1 Based on the chosen topic, complete the paper writing, select an SCI or SSCI Q1 journal, submit using my QRCID, and complete...

27 July 2024 8,965 1 View

Could someone please provide a list of journals that accept the application of methodologies in nature-based solutions?

I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.

23 July 2024 6,444 2 View

"Hello, I am trying to find public datasets containing FTIR spectra of blood samples (both healthy and disease-related)?

These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"

17 July 2024 6,519 3 View

You have mentioned as a co-author of one publicaitron?

You have mentioned as a co-author of one publication, but it is not exactly a publication , it is an abstract submitted to: Congresso Brasileiro de Hematologia, Hemoterapia e Terapia Celular -...

17 July 2024 4,035 15 View

Who wants opportunities for scientific cooperation?

Dear Colleagues, I hope this message finds you well. My name is Noor Al-Huda K. Hussein, and I am a researcher specializing in deep learning applications in genetic data analysis. I am currently...

16 July 2024 3,981 6 View