Can VSV proteins be shared among viruses?

Dear biologists, I am wondering when we co-infect insect cells with two viruses (BacMAM viruses), only one of them containing VSV gene. Would VSV-G protein integrate onto both viruses produced...

10 November 2017 1,886 0 View

How to improve PCR fidelity while using DMSO to overcome GC rich template PCR?

Hi friends, I am trying to clone some GC rich fragments. The PCRs work well while I use 5% DMSO in the reactions, hardly any band without DMSO. The problem is that the DMSO inclusion largely...

09 October 2017 6,420 3 View

Chromosome associated RNA (caRNA), where I can find database for none coding RNA including caRNA?

06 July 2017 7,182 1 View

No title

11 December 2016 6,295 1 View

Any anti monomeric EGFP antibody available?

06 July 2016 3,894 5 View

What special attention should I pay for purification of recombinant type II restriction EcoRV?

05 June 2015 1,556 4 View

Can ProA-immunoglobulins affinity be directly used for western?

I have a ProA tagged protein in yeast. I wonder if I can use yeast crude lysate in SDS PAGE, then visualize ProA by anti-mouse IgG-peroxidase conjugate as western analysis? Or I have to do it in a...

03 April 2015 5,188 1 View

How can we increase the solubility of E. coli overexpressed protein?

We are trying to purify an artificial protein. Very good expressed, but mostly in the spun down pellet. If anyone has experience to make it a bit more soluble, with a kind of mild buffer,...

31 December 2014 5,830 8 View

How can we perform migration of peptide correctly in SDS-PAGE?

11 December 2014 6,034 8 View

How can I decrease lysate viscosity for isolation of DNA/protein complex from cell disruption?

Dear all, I am going to isolate a DNA/Protein complex from insect cells. In protein purification practice, generally, we use nuclease during the cell disruption to destroy the released nucleic...

07 August 2014 3,391 6 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Why is My firend's profile not visible?

Hello, I created a profile for my friend on google scholar. I have added a verified email address. I marked the profile as public. Formerly his profile was visible. However, now, When I type his...

24 February 2021 8,680 3 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

I can't edit my publication, the "edit" is blurred. How can I edit it?

I have entered a wrong question mark in my publication and added "Forthcoming", I want to edit the title of my publication, but the "EDIT" is blurred, I can't edit it. Any suggestion? thank you.

18 February 2021 1,279 3 View

How to decrease the time for publication of paper in a journal ?

It takes a lot of time in publishing the paper in the journal, from few months to a year so how one can able to decrease the time for publication.

17 February 2021 2,997 14 View