Dear all,
I am trying to find some sequence information about chromosome associated RNA. Please may you let me know where should head? Thank you.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3013714/
Dear biologists, I am wondering when we co-infect insect cells with two viruses (BacMAM viruses), only one of them containing VSV gene. Would VSV-G protein integrate onto both viruses produced...
10 November 2017 2,087 0 View
Hi friends, I am trying to clone some GC rich fragments. The PCRs work well while I use 5% DMSO in the reactions, hardly any band without DMSO. The problem is that the DMSO inclusion largely...
09 October 2017 6,542 3 View
Dear friends, May you let me know how can I find out the transcription start site of gene CDKN1A from any database or any public resource? Thank you. ziguo
06 July 2017 2,359 2 View
Dear friends, Does anyone knows any Coli Strain available for plasmid with R6Kγ origin (pir gene) and with reduced recombination to increase plasmid stability? I am currently experiencing a very...
11 December 2016 6,427 1 View
Dear All, I am aftering anti-monomeric EGFP antibody for western blotting analysis. Any friend knows where I may buy such antibody, please? Thank you.
06 July 2016 4,019 5 View
Hi experts, I need a lot EcoRV restrict enzyme for routine usage. I prepare to make by my own. As restricts are not normal protein, do I need special tricks to deal with the over-expressed enzyme...
05 June 2015 1,672 4 View
I have a ProA tagged protein in yeast. I wonder if I can use yeast crude lysate in SDS PAGE, then visualize ProA by anti-mouse IgG-peroxidase conjugate as western analysis? Or I have to do it in a...
03 April 2015 5,317 1 View
We are trying to purify an artificial protein. Very good expressed, but mostly in the spun down pellet. If anyone has experience to make it a bit more soluble, with a kind of mild buffer,...
31 December 2014 5,954 8 View
Dear All, I am trying to detect synthesised peptides with SDS-PAGE. The peptides migrate with a big difference (larger) in SDS-PAGE gel in comparison with their real molecular weight. And they...
11 December 2014 6,171 8 View
Dear all, I am going to isolate a DNA/Protein complex from insect cells. In protein purification practice, generally, we use nuclease during the cell disruption to destroy the released nucleic...
07 August 2014 3,519 6 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...
09 August 2024 5,048 2 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View