Dear sir,

You need grouping variable and independent variables for PCA and DFA analysis in SPSS. If you have 2 populations/species take grouping variable as name of the population/species then put the corresponding independent variables against the grouping variable. For example...for two populations..

Population Varible 1 Variable 2 variable 3 variable 4

1 1.846163372 2.750819762 1.066226848 3.570076888

1 1.319154501 1.783463268 0.751887394 2.849958907

1 2.437551691 3.997620437 1.395285115 4.326324655

1 1.636695363 1.936954755 2.428583519 2.94560643

1 1.624042034 2.164522791 0.904074934 3.13419123

1 1.66323773 2.455149511 0.975777635 2.922877598

1 1.657815735 2.343986283 0.987542906 3.034960038

1 2.078424222 2.548837677 1.060420278 2.795921084

2 1.846163372 2.750819762 1.066226848 3.570076888

2 1.319154501 1.783463268 0.751887394 2.849958907

2 2.437551691 3.997620437 1.395285115 4.326324655

2 1.636695363 1.936954755 2.428583519 2.94560643

2 1.624042034 2.164522791 0.904074934 3.13419123

2 1.66323773 2.455149511 0.975777635 2.922877598

2 1.657815735 2.343986283 0.987542906 3.034960038

2 2.078424222 2.548837677 1.060420278 2.795921084

For variables given above you can use your parameters like DO, Alkalinity, or Organism length, height etc...

I hope this could enlighten you to some extent.

Regards

Javaid Iqbal

Well i,ll check it.... Thanks

Dear Ummer,

Use this Url

https://www.youtube.com/watch?v=UYxboC27190

Also, you can use:

https://www.youtube.com/watch?v=CzWEJLoNmLA

very useful training