For connection between the bolt to the steel element, which kind of interaction should be considered on ABAQUS?

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Marker gene metagenome sequencing?

The source of classification bias in marker gene metagenome sequencing? a variability in the taxonomy classification of microbial communities when using different primer pairs (e.g. for 16S rDNA)...

29 June 2024 1,048 1 View

fluorescent imaging without fixatives?

How to store SDS-PAGE for fluorescent imaging without fixatives? I am running protein samples on SDS-PAGE that are tagged with fluorophores. If I place the gel in a destain solution of...

29 June 2024 8,720 1 View

Is a 5` phosphate needed for homologous recombination?

Is a 5` phosphate needed for homologous recombination? I am a bit confused whether phosphorylated DNA is needed to achieve efficient homologous recombination in e coli. I believe there are methods...

06 June 2024 7,065 1 View

Diluting an enzyme before an experiment?

What is the reason or importance of diluting an enzyme before an experiment? I'm writing a report on enzyme activity and characterisation and i need help with understanding this: we were told to...

06 June 2024 8,714 2 View

How do you get Rid of Nonspecific Bands in PCR?

So, here is what I am dealing with: The Tm of the primers is 55 for the forward one and 56 for the reverse one. In -silico PCR produces 750 BP band, but in reality I get 3 bands. The third one...

17 March 2024 9,914 5 View

PCR nonspecific product?

I am amplifying target sequence 450 bp. I get single sharp band in the control and faint in sample with another sharp nonspecific product. why? I need to get one single band from my sample to...

17 March 2024 804 3 View

CRISPR targeting in cell line, flow or single clone?

I am doing a CRISPR targeting to knock out one intron of my gene of interest in a cancer cell line and replace that intron with GFP. I am wondering if I should pick single cell clone with possible...

17 March 2024 9,713 1 View

How convert Bacterial DNA concentration to copy numbers?

For example, If a PCR primer can detect ~100 fg of DNA, how we will calculate it to Bacterial copy number?

17 March 2024 1,816 2 View

Could you please introduce some reliable references about electrical properties and structure of semiconductors?

I would like to find a reliable reference that includes electronic properties such as CB & VB DoS, electron & hole mobilities, thermal velocities of semiconductors like ZnTe, CdTe. I am...

10 February 2024 7,803 4 View

Guide RNA for improved CRISPR-Cas9 gene editing ?

How can the design and synthesis of guide RNA molecules be optimized for improved CRISPR-Cas9 gene editing?

05 August 2023 1,375 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

How do environmental toxins interact with genetic predispositions to increase cancer risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 2,627 1 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

Will the catalytic effect of retained austenite happens in steels including RA?

The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...

27 July 2024 8,508 0 View

Please investigate the effect of temperature and strain rate of hot deformation and the formation of delta ferrite in martensitic stainless steel 410?

25 July 2024 9,408 0 View

How to saturate steel samples with hydrogen?

I have tried to saturate ferritic steel with hydrogen using the electrolytic method and H2SO4. However, the effect was very poor even after 3 days of "saturation". Can anybody help in choosing a...

24 July 2024 4,393 2 View

I am working on III-V based tandem solar cells.Can anyone explain that solar cells work under forward or reverse biased conditions?

I want to know that n-doped side of solar cell is connected with positive or negative electrode.

20 July 2024 1,348 2 View