How to analyze result obtained from ddPCR using cDNA as template?

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MaríaIgnacia Meza Cerda

Hi there, did you answer your question?

If you did so, could you share with me how you analysed the data? I am having the same doubts.

Many thanks

James John

you know the template concentration you added in reaction mixture , just say 1 ul (and now if your RNA concentration was 10ng/ul), means you added 10 ng in reaction mixture.....now you have results that say lets 10 copies/ul in reaction mixture then you are able to calculate it to copies number/per reaction. this number indicate copies/10 ng of RNA......