MaríaIgnacia Meza Cerda

4 Questions 8 Answers 0 Followers

Questions related from MaríaIgnacia Meza Cerda

What is the lowest yield of RNA that can be measured (for integrity) on a Denaturing Agarose gel?

Hi everyone, After RNA extractions and DNase treatments, I measured my samples for RNA yields in NanoDrop. On average they are 10ng/ul (ranging from 2ng/ul to 29ng/ul). Very low. 260/280 ratio...

24 January 2019 7,295 1 View

How to precipitate RNA and concentrate it??

Hi everyone, I have extracted RNA from whole blood store in RNAlater for some years now. I have been using the RiboPure blood kit and adding two DNase treatments to get rid of gDNA. The kit...

23 January 2019 6,822 3 View

Anyone with a protocol for using Bead Beating for whole blood store in ARN later??

We are working with samples stored in ARNlater from some years now, and they look a little bit aggregated. I've been suggested to use glass beads, the big ones 1-4mm, to homogenize the samples...

21 August 2018 6,622 10 View

We are extracting RNA from blood (plasma taken out) in RNAlater for RT qPCR. Any suggestions of an easy/cost effective way to remove heparin?

Hi! I am trying to extract ARN from blood samples kept in ARNlater (plasma has been taken out in the field). Some of the samples where taken with EDTA and some with LiHeparin. It is no clear which...

09 August 2018 567 2 View