Dear all,
I have been doing retinal flatmounts from both mice and rats. For vasculature visualisation I have stained them with lectin FITC conjugated (image attached).
I am trying to find ways to best analyse vascular proliferation or angiogenesis in these samples.These can be in the form of length, number of branches, or any other relevant parameters.
Thanks in advance for any suggestions.
Arthur
Somethin useful might be found here:
https://www.nature.com/articles/nprot.2010.130
Hi,
You could quantify the vascular coverage (vascular area / flat mount area) or avascular area (depending what you want to show) and the number of branch points per mm².
As you have fluorescent pictures, you can easily analyse your samples by thresholding with any software (such as Fiji - ImageJ) and determine areas / branch points / network...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4819398/#!po=7.33333
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