to be used in 96 well plates.
Hi All! Looking for best practices in making stock mM EtOH and Paracetemol concentrations. Here is what I need for both 5, 10, 20, 30 and 40mM drug stock concentrations. I will be adding 10uL...
11 December 2017 4,366 0 View
With regard to HepG2 cells, does anyone know truly what the real expression of cyp2e1 is, if any? I am trying to establish a reliable dose response study of EtOH and APAP in vitro and want a cell...
11 December 2017 542 3 View
Making mM Ethanol concentrations? Hi All! Looking for best practices in making stock mM EtOH and Paracetemol concentrations. Here is what I need for both5, 10, 20, 30 and 40mM drug stock...
11 December 2017 2,376 0 View
Does anyone have any good methodology or techniques/ protocols to share on how to setup dose response curves properly.
08 September 2017 2,920 7 View
I am trying to put together dose response curves for both EtOH and Paracetamol using HepG2 cells and am unsure if they express CYP2E1. Some background seems to say they do not (MV5? cells). If...
08 September 2017 4,571 2 View
Hi, I am trying to conduct a dose response curve using HepG2 cells on 3D 96 well titer plate (alvonex) and am trying to remember how to use the hemocytometer. I want to seed 5000 cells/well but...
05 June 2017 9,215 1 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...
01 August 2024 2,250 2 View
Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...
01 August 2024 6,134 2 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View
Aim is to simulate the shape of Mobius strip (an elastic ribbon) in simple way ( using Abaqus Learning edition). In literature, Mobius strip is modeled as Kirchhoff Rod / Cosserat plate. But, such...
15 July 2024 4,388 1 View
How to calculate CFU/m3 in settle plate?
14 July 2024 1,816 1 View
