7 Questions 8 Answers 0 Followers
Questions related from Antonio Bifero
With regard to HepG2 cells, does anyone know truly what the real expression of cyp2e1 is, if any? I am trying to establish a reliable dose response study of EtOH and APAP in vitro and want a cell...
12 December 2017 527 3 View
Hi All! Looking for best practices in making stock mM EtOH and Paracetemol concentrations. Here is what I need for both 5, 10, 20, 30 and 40mM drug stock concentrations. I will be adding 10uL...
12 December 2017 4,326 0 View
Making mM Ethanol concentrations? Hi All! Looking for best practices in making stock mM EtOH and Paracetemol concentrations. Here is what I need for both5, 10, 20, 30 and 40mM drug stock...
12 December 2017 2,357 0 View
to be used in 96 well plates.
10 October 2017 3,145 0 View
I am trying to put together dose response curves for both EtOH and Paracetamol using HepG2 cells and am unsure if they express CYP2E1. Some background seems to say they do not (MV5? cells). If...
09 September 2017 4,557 2 View
Does anyone have any good methodology or techniques/ protocols to share on how to setup dose response curves properly.
09 September 2017 2,899 7 View
Hi, I am trying to conduct a dose response curve using HepG2 cells on 3D 96 well titer plate (alvonex) and am trying to remember how to use the hemocytometer. I want to seed 5000 cells/well but...
06 June 2017 9,195 1 View
