Does anyone have any good methodology or techniques/ protocols to share on how to setup dose response curves properly.
A curious question. What are you asking beyond plotting dose on one axis and response on the other?
Most important is that the uncertainty in the dose and the uncertainty in the response are smaller than the plotted quantity. Too much uncertainty will make the resulting prediction too uncertain to be meaningful.
@Bifero I guess this is for a radiation detector. If so, then you only plot a graph between delivered dose and detector (output-background reading). Use Cs or Co gamma source for dose delivery. Do the following:
Deliver doses in various ranges say from mGy to Gy or higher
GO on writing the output as well as BG
First Column: delivered dose
2nd column : detector output (say 3 readings for one dose)-average BG.
Plot graph X-axis delivered dose
Y-axis-output
and analyse further.
Thank you both so very much for your wonderful answers. I guess I should clarify I'm setting up those response curve for the concomitant use of alcohol and acetaminophen in Hep G2 cells essentially going to titrate ethanol on the y-axis and acetaminophen on the x-axis the 96 volt charger plate. I guess I'm having difficulty trying to figure out what would be the appropriate Target concentrations to use that would reflect the appropriate in Vivo concentrations of alcohol and Tylenol seen in the patient population. This is a peach tea project I'm working on, once I establish reproducible dose response curve that I'm going to try to figure out what the molecular details are
It is not easy to provide suggestions without appropriate information. What information do you have on in vivo concentrations?
From what little data i can find, roughly 15g apap=4nmol in 70kg adult. I guess my real question is when I'm titrating the drugs on the 96 well titer plate, I'm going to put the ethanol concentrations on the y axis and the acetaminophen concentrations on the x-axis and measure the response as cell viabilty through MTT. Does that make sense?
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