How the structures and composition of different types of meat (red and white) influence DNA extraction?

More Asmaa Maaz's questions See All

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View

What is the most accurate way to determine the energy gap of semiconductor materials? Is it better to use UV-Vis or PL measurements?

When preparing hierarchical zinc oxide ananostructure, the energy gap measured by PL was equal to (3.26 eV) While the energy gap value was determined by UV-Vis measurements (3.5 to 3.7 eV)

26 June 2024 7,505 6 View

What is the most accurate artificial urine recipe used in vitro for microbiological purposes?

I just found many protocols but not specific for bacterial growth

30 May 2024 6,305 2 View

How can I explain the acid base titration test for a PH responsive nanogel?

I have three nanogels.. The difference between them is the cationic monomer ratio... However, the test is giving me similar curve of the three formulations

27 May 2024 2,064 0 View

Question about XRD?

Hello, I was elaborating thin films from FeCl3 using a dip coater. These films were deposited on a glass sheet for 6 layers and then annealed at 500°C. However, XRD analysis did not yield any...

31 March 2024 9,413 3 View

Can I use two IRIS in the same plasmid expression cassette ?

I am trying to express m6A protein and a fluorescence one RFP in the piggyback vector. I was thinking first of using the P2A to separate between the m6A and the RFP protein but then I was afraid...

12 March 2024 9,713 0 View

How can I encapsulate cisplatin into a ph responsive polymeric nanogel?

I have synthesised a 100 nm sized cationic nanogel and I want to use it as a carrier for cisplatin and paclitaxel

03 March 2024 3,426 0 View

What tools needed to conduct areserch study about "burden of mothers in caring for child with colstomy" besides zarit burden interview scale ?

i'm preparing my protocol for master degree and it's my first time ,i need some help ,my reserch titel is "burden of mother in caring for child with colstomy"

02 March 2024 5,656 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

What are the steps of formulating a herbal extract into a sheep/goat drench?

I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...

30 July 2024 9,806 1 View

Why not cow dung manure used in lie for artificial ?

Cow dung manure is more powerful than artificial fertilizer.

19 July 2024 7,392 7 View

Is cow dung can be in used for repeated use ?

Is this organic cow dung manure now important than artificial fertilizer.if good then why ?

19 July 2024 9,052 2 View

How can I analyze thermal camera images to measure heat stress in dairy cows?

Hi, I am working on heat stress in dairy cows. In a recent experiment, we used a FLIR-T62101 thermal camera to take photos of the cows. One photo is attached. How can I analyze these images to...

30 June 2024 2,190 5 View

Could anyone recommend antibodies to TET2, TET3 and DNMT3B for bovine?

Can anyone recommend polyclonal or monoclonal antibodies for Tet methylcytosine dioxygenase 2 (TET2) Tet methylcytosine dioxygenase 3 (TET3) DNA methyltransferase 3 beta (DNMT3B) Ideally, they...

27 June 2024 3,557 1 View

Where can I find my citations for my published papers?

1- M.M.M. Ahmed, F.M. El Hag, F.S. Wahab, S.F. Salih (2001). Feeding strategies during dry summer for lactating desert goats in a rainfed area under tropical conditions. Small Ruminant Research 39...

18 June 2024 552 5 View

Litopenaeus vannamei post-larvae for fatty acid and amino acid profile?

For fatty acid and amino acid profile analysis in Litopenaeus vannamei post-larvae (1-1.5 gr), is it necessary to remove the head and peel? or it's possible to use whole body including head, peel...

04 June 2024 4,497 0 View

Oral chicken Candida infection?

What you know about oral chicken Candida infection?

31 May 2024 9,042 1 View

I'm facing serious problem about sample size determination please help me?

I'm doing research in veterinary science and topic is associated with ticks prevalence and seasonal variation in cow..I have randomly selected 560 cows as sample size and studing for prevalence...

25 May 2024 3,834 5 View

Khalid Hassan

Dear Asmaa

Please, read in the following link:

https://www.researchgate.net/publication/276509768_Fast_and_efficient_extraction_of_DNA_from_meat_and_meat_derived_products_using_aqueous_ionic_liquid_buffer_systems

Good Luck

Article Fast and efficient extraction of DNA from meat and meat deri...

Goran Kušec

Dear Asmaa, we tested some commercial kits againts traditional method of DNA isolation... maybe can be of some help:

https://www.researchgate.net/publication/283242221_Comparison_of_commercial_DNA_kits_and_traditional_DNA_extraction_procedure_in_PCR_detection_of_pork_in_dryfermented_sausages

Article Comparison of commercial DNA kits and traditional DNA extrac...