In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

What is the best protocol to fix cell in order to visualize the membrane by Scan electron microscopy (SEM)?

Hi, I want to study the membrane using SEM, I am having some problems with cell fixation. I am using PFA and glutaraldehyde , and one lasts step with HDMS ( hexamethyldisilazane). But I think the...

21 November 2019 408 3 View

Anyone with experience coating with Hexamethyldisilazane by PECVD?

The functionality of the coating would be a gas barrier, on plastic substrate. Has anyone had experience using Hexamethyldisilazane as precursor in PECVD? Do I need a vaporizer to use this...

07 August 2019 1,779 5 View

PECVD using hexamethyldisilazane (HMDSN) as precursor, Is a vaporizer needed?

I'm using a home-made PECVD processing chamber with parallel plate/showerhead reactor. I may add reactive and carrier gases as needed, but I don't know details about feeding hexamethyldisilazane...

03 April 2019 1,793 2 View

Adhesion of photoresist(AZ-5214e)?

Does it work to add HMDS on ZnO thin film to improve the adhesion of photoresist? In addition to HMDS, is there other way to increase the adhesion of photoresist coated on ZnO thin film?

02 March 2019 6,219 3 View

Preparation of Metal Naphthalocyanines?

I have some doubts regarding the synthesis of naphthalocyanines. I have seen several methods for preparing Naphthalocyanines like the following: i) treating the naphthalonitrile with a base...

28 February 2019 184 2 View

Is it natural that bubbles are created during thick photoresist(SU-8) film baking?

09 October 2018 617 0 View

Can HMDS, LOR and PPR be used together in a recipe?

Hello all I have an oxide surface whom I want to pattern and later need metal deposition on the patterned surface (lift-off). I have used bilayer method i.e LOR (LOR 3B) and PPR (S1813) coating...

08 September 2018 4,666 3 View

What are gas sensor testing labs?

I'm looking for some labs that can evaluate gas absorbent pads used to protect gas sensors. The target gased to be absorbed are H2S, SO2, and HMDS. Thanks!

08 September 2018 2,671 1 View

How I can make glass surfaces hydrophobic for Langmuir-Blogett films?

I need to make Langmuir-Blodgett films, and for this I need hydrophobic substrates of glass. How I can do this ?? I know that the ferric stearate and hexamethyldisilazane are good options to...

14 August 2018 632 3 View

Why is az 4620 photoresist on Silicon stripping off?

Hello! I'm Hyunsu from hanyang university in Korea. I have a problem with stripping off of az 4620 photoresist on Silicon when mask and photoresist is detached. Photolithography process was done...

04 May 2018 5,075 3 View