Hello,

The other method I think about is lyophilization or freeze drying but it is demanding some equipments and the use of liquid nitrogen.

HMDS method just needs HMDS. HMDS is an inexpensive chemical easy to use under hood. At the end, if you are not in a hurry, you just let it evaporate under the hood over night, otherwise oven.

What are your specimens? For biological tissue I would recommend obtaining (as already did Nicolas Barois ) some HMDS, inexpensive and sold by nearly all EM suppliers.

Thank you sir Nicolas and Vladimir, your valuable suggestions

Sir, My specimen is a fish gill @Vladimir

Sir @Nicolas, @Vladimir

presently i am using liquid nitrogen for the purpose.

Any suggestion regarding this ?

I wanted to know, after dehydration process can we skip this drying process and proceed for the next ?

Proper dehydration wont work fir the same ?

dehydration = replacement of water by solvent (ethanol, acetone).

If you dry by evaporation of the solvent, you will obtain a very bad quality of your sample structure.