Recently I observed that my samples containing human AAT loose its concentration when stored at -20oC.
Does anybody have an experience in safe conditions for storing AAT samples- how long and in what temperature are they stable?
Have you tried adding glycerol to the sample to prevent freezing? Then perhaps storage at -80C would even work. My guess is the A1AT is denaturing during freezing.
Hi Ewa, a1AT should be stable at any temperature unless there is thrombin or other proteinases present.
a1AT has a reactive center loop that is a bait for proteinases. If you are unsure whether there are proteinases in your sup, then store the samples at -80oC. Serine proteinases are still active at -20oC.
Thank you for your answers.
I already moved my samples to -80C and I will observe the outcome.
I have got new information about my AAT protein that I still wanted to share with you. My standard protein was from Sigma and I got to know that it contains impurities making it polymerize, and therefore the concentration would drop.
AAT when stored in sterile conditions should be very stable.
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