Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Best rat model of Type 2 diabetes and metabolic syndrome?

Hi Im seeking advice on what would be the best model of T2D and metabolic syndrome in rats Thank you

04 September 2020 3,661 4 View

How can I digest the brain tissue in healthy mice as evaluating the apoptosis using flow cytometery by annexin V/propidium iodide (PI)?

We digested the brain tissue with Trypsin ( 1x) for 15 min (all steps were performed in cold conditions) and suspend the cells by pipetting up and down (3-4 times) to the single-cell suspension....

22 August 2020 5,611 4 View

Hi, is there anyone tell me or share me a link for downloading the template for "Cities" journal of Elsevier?

I went through the cities journal website but unluckily couldn't find the template

28 June 2020 811 3 View

Can anyone suggest a kit or protocol to extract Brucella spp. DNA from FFPE blocks?

Hi everyone! I am trying to extract Brucella DNA from FFPE blocks. Can anyone suggest me any good kit or protocol? I checked many articles and kits but still could not find an appropriate kit or...

15 December 2019 9,767 2 View

Photovoltaics are solar energy, renewable and economical. Why are people not very attracted by the benefits of domestic use of this green energy ?

Hello, Photovoltaics are solar energy, renewable and economical. Why are people not very attracted by the benefits of domestic use of this green energy ? Thank you

06 April 2019 8,782 19 View

What is the contribution of renewable energies in the fight against global warming?

The phenomenon of the greenhouse effect is a threat to the environment. What is the contribution of renewable energies in the fight against global warming?

04 April 2019 1,239 33 View

Methodology for implementing a thesis ?

Methodology for implementing a thesis involves: - State of the art of the studied phenomenon - Study context - Introduction - Hypotheses - Objectives - Labor interest - Methodology apprehended -...

14 February 2019 6,502 16 View

The passage from micro to nano scale in indentation of materials is of concern to researchers. How does one proceed to arrive at relevant answers?

The passage from micro to nano scale in indentation of materials is of concern to researchers. How does one proceed to arrive at relevant answers?

24 January 2019 5,584 2 View

How is 3D pelvic kinematics examined during walking before and after interventional exercises in patients with pelvic inclination?

in general, for healthy people, in the 3D motion analysis to define the anatomical coordinate system on segments, one frame in static position is taken by the camera, which will make segment...

10 October 2018 4,098 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View