It is well known that cells that are Propidium Iodide (PI) -ve and annexin V (AV) -ve are alive cells; PI-ve AV +ve are early apoptotic cells; PI+ve AV+ve late apoptotic cells and PI+ve AV-ve are damaged cells (necrotic cells). So, does this apply for the staining with LIVE/DEAD and AV?
Attached is an example of staining with LIVE/DEAD and AV.