I think you can use the Dead Cell Removal Kit from Miltenyi.

https://www.miltenyibiotec.com/US-en/products/macs-sample-preparation/removal-reagents/dead-cell-removal-kit.html

I use this on intestinal lamina propria lymphocyte prep for scRNA seq downstream application. It is quite effective and relatively fast.

you can also run the cells through the column twice if the first run doesn't give you desired target viability.

good luck!

Dead cell removal kit, MACS columns, ficoll or lymphoprep. In principle all of these work.

P.S. I woud centrifuge at 100 g for 10 min without brakes in after suspending the cells in 2% FCS in PBS.

Hi Aaron Christensen-Quick

I have selected primary human lymphatic endothelial cells with lentivirus mediated puromycin selection marker. In my case, after selecting the cells in presence of puromycin the negative cells or dead cells detach from the dish. Using multiple PBS wash and expanding the selected cells usually help get rid of dead or negative cells. I do not know how adherent your T cells are. But you may try multiple washing with PBS. Thank you.

Thank you for your comments! Riaj, unfortunately T cells are suspension cells. I see StemCell has a negative selection dead cell removal kit as well. I'll update this post with results.