Aaron Christensen-Quick

2 Questions 3 Answers 0 Followers

Questions related from Aaron Christensen-Quick

What are good co-culture conditions to measure target-specific T cell killing of a cell line? How should I detect the cytolysis?

Hi there, I'm trying to detect the specific killing of target cells (a CHO-based cell line) by genetically modified T cells. The primary human T cells (both CD4 and CD8) were modified to express a...

24 January 2019 4,625 0 View

How should I purify/isolate virally transduced T cells after selection and expansion?

I am trying to generate a pure population of lentivirally transduced primary human CD4 and CD8 T cells. My main obstacle at this point is the last purification step. I have already successfully...

21 January 2019 8,279 4 View